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mycobacteria selective medium middlebrook 7h9  (MiddleBrook Pharmaceuticals)

 
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    MiddleBrook Pharmaceuticals mycobacteria selective medium middlebrook 7h9
    Mycobacteria Selective Medium Middlebrook 7h9, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/selective+middlebrook+medium/pmc08458382-144-19-22?v=MiddleBrook+Pharmaceuticals
    Average 90 stars, based on 1 article reviews
    mycobacteria selective medium middlebrook 7h9 - by Bioz Stars, 2026-06
    90/100 stars

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    Pulmonary bacterial burden was evaluated in the lungs of bone marrow chimeric mice at 8 (A) and 14 (B) weeks following Mtb infection. NH-TLR2KO, H-TLR2KO, and TLR2KO chimeric mice were compared to WT mice. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of lung homogenates onto <t>7H11</t> agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU in the lungs. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01 *Denotes significance as compared to WT. Data is representative of two independent experiments with similar results. Expression levels of Ifnγ and Tnf in the lungs of 8 week infected mice (C) were determined by isolation of total lung RNA followed by RT-PCR analysis. PCR data includes 5 mice per group. All data is represented as mean ± SEM. Data is representative of two independent experiments. Significance was determined by unpaired T-test. *p< 0.05.
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    Pulmonary bacterial burden was evaluated in the lungs of bone marrow chimeric mice at 8 (A) and 14 (B) weeks following Mtb infection. NH-TLR2KO, H-TLR2KO, and TLR2KO chimeric mice were compared to WT mice. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of lung homogenates onto <t>7H11</t> agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU in the lungs. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01 *Denotes significance as compared to WT. Data is representative of two independent experiments with similar results. Expression levels of Ifnγ and Tnf in the lungs of 8 week infected mice (C) were determined by isolation of total lung RNA followed by RT-PCR analysis. PCR data includes 5 mice per group. All data is represented as mean ± SEM. Data is representative of two independent experiments. Significance was determined by unpaired T-test. *p< 0.05.
    Middlebrook Selective Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Pulmonary bacterial burden was evaluated in the lungs of bone marrow chimeric mice at 8 (A) and 14 (B) weeks following Mtb infection. NH-TLR2KO, H-TLR2KO, and TLR2KO chimeric mice were compared to WT mice. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of lung homogenates onto 7H11 agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU in the lungs. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01 *Denotes significance as compared to WT. Data is representative of two independent experiments with similar results. Expression levels of Ifnγ and Tnf in the lungs of 8 week infected mice (C) were determined by isolation of total lung RNA followed by RT-PCR analysis. PCR data includes 5 mice per group. All data is represented as mean ± SEM. Data is representative of two independent experiments. Significance was determined by unpaired T-test. *p< 0.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Divergent functions of Toll-like receptor 2 on hematopoietic and non-hematopoietic cells during chronic Mycobacterium tuberculosis infection ‡

    doi: 10.4049/jimmunol.1601651

    Figure Lengend Snippet: Pulmonary bacterial burden was evaluated in the lungs of bone marrow chimeric mice at 8 (A) and 14 (B) weeks following Mtb infection. NH-TLR2KO, H-TLR2KO, and TLR2KO chimeric mice were compared to WT mice. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of lung homogenates onto 7H11 agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU in the lungs. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01 *Denotes significance as compared to WT. Data is representative of two independent experiments with similar results. Expression levels of Ifnγ and Tnf in the lungs of 8 week infected mice (C) were determined by isolation of total lung RNA followed by RT-PCR analysis. PCR data includes 5 mice per group. All data is represented as mean ± SEM. Data is representative of two independent experiments. Significance was determined by unpaired T-test. *p< 0.05.

    Article Snippet: The stock titer was determined by plating 10-fold serial dilutions on Middlebrook 7H11 selective medium (Difco BD, Franklin Lakes, NJ).

    Techniques: Infection, Incubation, Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction

    Bacterial burden in the spleen, liver, and mediastinal lymph nodes (MLN) was evaluated in bone marrow chimeric Mtb-infected mice at weeks 8 and 14. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of homogenates onto 7H11 agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01. *Denotes significance as compared to NH-TLR2KO. Data are representative of two independent experiments with similar results.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Divergent functions of Toll-like receptor 2 on hematopoietic and non-hematopoietic cells during chronic Mycobacterium tuberculosis infection ‡

    doi: 10.4049/jimmunol.1601651

    Figure Lengend Snippet: Bacterial burden in the spleen, liver, and mediastinal lymph nodes (MLN) was evaluated in bone marrow chimeric Mtb-infected mice at weeks 8 and 14. Time points were performed after separate aerosol infections with 4–5 mice used per group. The number of viable bacteria was determined by plating serial dilutions of homogenates onto 7H11 agar plates. Colonies were counted 21 days after incubation at 37°C to determine CFU. Data are presented as mean CFU counts ± SEM. Significance was determined by Bonferroni Multiple Comparisons test of One-Way ANOVA. *p<.05; **p<.01. *Denotes significance as compared to NH-TLR2KO. Data are representative of two independent experiments with similar results.

    Article Snippet: The stock titer was determined by plating 10-fold serial dilutions on Middlebrook 7H11 selective medium (Difco BD, Franklin Lakes, NJ).

    Techniques: Infection, Incubation